Journal: Endocrinology

Article Title: Intestinal Flora is a Key Factor in Insulin Resistance and Contributes to the Development of Polycystic Ovary Syndrome

doi: 10.1210/endocr/bqab118

Figure Lengend Snippet: Intestinal FXR plays an important role in glucose metabolism in PCOS model mice . Mice were divided into 3 groups (LET, LET+CDCA, and LET+GUDCA) and were treated for 35 days to explore the effects of intestinal FXR (LET: 4, LET+CDCA: 7, and LET+GUDCA: 8). (A) Schematic representation of the experimental design. (B) GTT. (C) Fasting blood glucose. (D) Thirty minutes after glucose injection. (E) Mean blood glucose. (F) Area under the curve (AUC) of the GTT. (G) Fasting insulin. (H) HOMA-IR. (B-H) P values were determined by 1-way ANOVA with the least significant difference (LSD) multiple comparison post hoc test. P < 0.05 was considered statistically significant. CDCA, chenodeoxycholic acid; FXR, farnesoid X receptor; GTT, glucose tolerance test; GUDCA, glycoursodeoxycholic acid; HOMA-IR, homeostasis model assessment for insulin resistance index; LET, letrozole; PCOS, polycystic ovarian syndrome.

Article Snippet: Mice in the LET+CDCA group were administered LET and CDCA (Targetmol, catalog #T0847) at a dosage of 50 mg/kg/d ( ) once daily by oral gavage.

Techniques: Injection, Comparison

Journal: iScience

Article Title: Hepatic Lamp2a deficiency promotes inflammation of murine autoimmune cholangitis via affecting bile acid metabolism

doi: 10.1016/j.isci.2025.111804

Figure Lengend Snippet:

Article Snippet: Chenodeoxycholic acid(CDCA) , GLPBIO , Cat#GC17985.

Techniques: Purification, Virus, Recombinant, Staining, Adjuvant, Cell Stimulation, Labeling, Isolation, Enzyme-linked Immunosorbent Assay, Software

Journal: Journal of Lipid Research

Article Title: Parenteral lipids shape gut bile acid pools and microbiota profiles in the prevention of cholestasis in preterm pigs [S]

doi: 10.1194/jlr.RA120000652

Figure Lengend Snippet: Bile acid concentrations in gallbladder contents of preterm piglets after 22 days

Article Snippet: The free and conjugated bile acid concentrations in samples were determined by reverse isotope dilution methodology using internal standards of d9-chenodeoxycholic acid (CDCA) and d9-glyco-CDCA (Cambridge Isotopes, Andover, MA).

Techniques:

Journal: Journal of Lipid Research

Article Title: Parenteral lipids shape gut bile acid pools and microbiota profiles in the prevention of cholestasis in preterm pigs [S]

doi: 10.1194/jlr.RA120000652

Figure Lengend Snippet: Colonic contents bile acid profiles and relative ratios of conjugated to unconjugated bile acids. A: Heatmap showing profiles of bile acid species detected in samples from colon contents, with bile acid species arranged by hierarchical clustering. Values are relative standardized units (dimensionless). B: Relative ratios of total conjugated (both glyco- and tauro-conjugated) to unconjugated chenodeoxycholate (CDCA), hyocholate (HCA), and hyodeoxycholate (HDCA) found in colon contents samples. Values are ratios of relative standardized units (dimensionless). Bars represent standard error. *P < 0.05 versus ENT, #P < 0.05 versus IL (Holm-adjusted pairwise t-tests).

Article Snippet: The free and conjugated bile acid concentrations in samples were determined by reverse isotope dilution methodology using internal standards of d9-chenodeoxycholic acid (CDCA) and d9-glyco-CDCA (Cambridge Isotopes, Andover, MA).

Techniques:

Journal: JCI Insight

Article Title: Lipidomics unveils lipid dyshomeostasis and low circulating plasmalogens as biomarkers in a monogenic mitochondrial disorder

doi: 10.1172/jci.insight.123231

Figure Lengend Snippet: (A–D) LC-QTOF–based untargeted lipidomics from livers of fed H-Lrpprc–/– mice (n = 13) and their littermate controls (n = 8). (A) Volcano plot depicting the 1,386 features obtained following MS data processing. Using a P-corr threshold of 0.05 (corresponding to an uncorrected P value of 0.015; horizontal red dotted line) and an FC >2.5 or <0.4 (vertical red dotted lines), 92 features significantly discriminated H-Lrpprc–/– mice vs. controls, of which 60 were increased (red dots) and 32 decreased (green dots). See also Supplemental Table 3 for the list of lipids identified by MS/MS with FCs and P values (unpaired Student’s t test and with Benjamini-Hochberg correction). (B–D) Dot plots of selected lipids significantly discriminating H-Lrpprc–/– mice from controls and identified by MS/MS. Each dot represents a log2-transformed signal intensity ratio for the indicated lipid (sub)classes with their acyl side chain(s): (B) 15 glycerolipids (TGs); (C) 22 glycerophospholipids: 9 PC, 9 PE, 2 phosphatidylglycerol (PG) and 2 phosphatidylserine (PS); and (D) 4 ACs.

Article Snippet: Briefly, plasma samples or pulverized liver tissues previously freeze-clamped (20 mg) were homogenized in methanol/water (80:20, v/v) spiked with deuterated standards ([ 2 H 4 ]lithocholic acid, d 4 -LCA; [ 2 H 4 ]cholic acid, d 4 -CA; [ 2 H 4 ]chenodeoxycholic acid, d 4 -CDCA; [ 2 H 4 ]deoxycholic-2,2,4,4-d 4 acid, d 4 -DCA); [ 2 H 4 ]glycodeoxycholic acid, d 4 -GDCA; [ 2 H 4 ]glycochenodeoxycholic acid, d 4 -GCDCA; and [ 2 H 4 ]glycocholic acid, d 4 -GCA) (CDN Isotopes) and then acidified with HCl (1 mM) before homogenization and centrifugation.

Techniques: Tandem Mass Spectroscopy, Transformation Assay